Any Drive 32 GB or larger is supported by Medicat.
We strongly suggest using a USB 3.0 or above as using USB 2 will severely dampen the speed of your install and some tools may fail to boot.
That being said Medicat will boot with computer that only has 2.0 slots.
Matt And Jayro strongly suggest using a USB SSD enclosure if you plan to use Medicat on a semi regular basis.